Plenary Lecture
Oncoproteomics: Challenges, Techniques and Possibilities to Cancer Biomarker Discovery
Professor Shaden Muawia Hanafy
Genetic Engineering and Biotechnology Research Institute
Department of Molecular Biology
University of Minofya
Egypt
E-mail: shadenmuawia@yahoo.com
Abstract: Proteomics is the large-scale study of the structure and function of proteins in complex biological sample. Such an approach has the potential value to reveal the complexity and heterogeneity of the human proteome. In addition, proteomics has been complemented by the analysis of posttranslational modifications and techniques for the quantitative comparison of different proteomes..Oncoproteomics is the study of proteins and their interactions in a cancer cell by proteomic technologies and parallels the related field of oncogenomics. There is an intense interest in applying proteomics to improve understanding of cancer pathogenesis, develop new tumor biomarkers for diagnosis, and early detection using proteomic portrait of samples. In pace with the successful completion of the Human Genome Project, the wave of proteomics has raised the curtain on the postgenome era. The study of oncoproteomics provides mankind with a better understanding of neoplasia. . It is now contributing to the development of personalized management of cancer. In recent years, substantial technological advances in proteomics have permitted the quantitative analysis and identification of protein changes associated with cancer. Tumor-associated biomarkers that can be determined via proteomic approaches represent a subset of possible cancer targets. To achieve this, several proteomic approaches including two-dimensional polyacrylamide gel electrophoresis, mass spectrometry and protein microarray have been developed and are being used in different combinations. This review provides an overview of the proteomic approaches available for cancer target identification and reviews recent analyses of protein expression in various cancers.
Brief Biography of the Speaker: Dr Sh. Muawia holds a BSc in Biochemistry from Faculty of Science- Alexandria University-Egypt. She holds a Master degree & a PhD in Biochemistry (1992, 2001 respectively) from the Faculty of Science- Alexandria University. She works as a Professor of Biochemistry and Molecular Biology in the Molecular Biology department in Genetic Engineering and Biotechnology research Institute (GEBRI) - Minofya University- Sadat City. Her research interests include: Apoptotic signalling pathway and apoptotic markers in different tumors (adult acute myeloid leukemia and in Hepatocellular Carcinoma) and in HCV patients. Single nucleotide polymorphism and gene expression of different genes in various diseases and tumors. Oxidative stress and antioxidant defence beside cytokine expression on the molecular and protein levels. She has 30 publications in highly rated ISI journals and attends 28 conferences.