AUTHORS: Mražíková Anna, Velgosová Oksana, Kavuličová Jana, Málek Jaroslav, Krum Stanislav

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ABSTRACT: Unique physicochemical properties of silver nanomaterials dependent not only on the shape and size of silver nanoparticles (AgNPs), but also on the surface capping agent. This study provided comparison of antimicrobial activities of chemically synthesized AgNPs and biosynthesized AgNPs against green algae on agar plates and also in the aqueous environment. The extract of the green freshwater algae Parachlorella kessleri acted both as a reducing as well as stabilizing agent during the formation of AgNPs. For chemically synthesized AgNPs, citrate and gelatin were used as a reducing and capping agent, respectively. The formation of AgNPs was confirmed by UV-Vis and TEM measurements. UV-Vis results revealed that both used Bio-AgNPs and Chem-AgNPs exhibited long-term stability. Antimicrobial activities of Bio-AgNPs and Chem-AgNPs were examined against green algae P. kessleri using disk diffusion methods. Comparing the antimicrobial activity on agar plates of Bio-AgNPs and Chem-AgNPs according to the zone inhibition around swabs showed stronger toxic effects of Bio-AgNPs. On the other hand, Bio-AgNPs were confirmed to be less toxic in aquatic environments for the growths of green algae P. kessleri comparing to Chem-AgNPs where cells growth reduction after 30 h was 60% in the presence of Chem-AgNPs

KEYWORDS: silver nanoparticles, antibiofilm activity, biosynthesized nanoparticles, chemically synthesized nanoparticles

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